Research banner

Centre for

Marine Biodiversity and Biotechnology

From the shoreline to the deep ocean, tackling issues from pure ecology
and physiology to aquaculture, marine spatial planning and conservation.


Chromatography involves a sample (or sample extract) being dissolved in a mobile phase (which may be a gas, a liquid or a supercritical fluid). The mobile phase is then forced through an immobile, immiscible stationary phase. The phases are chosen such that components of the sample have differing solubilities in each phase. A component which is quite soluble in the stationary phase will take longer to travel through it than a component which is not very soluble in the stationary phase but very soluble in the mobile phase. As a result of these differences in mobilities, sample components will become separated from each other as they travel through the stationary phase.

Within CMBB we have the following facilities:

  • High Performance Liquid Chromatography: chromatographic technique used to separate a mixture of compounds in analytical chemistry and biochemistry with the purpose of identifying, quantifying and purifying the individual components of the mixture
  • Fast Protein Liquid Chromatography: form of liquid chromatography used to separate or purify proteins from complex mixtures
  • Gas Chromatography (GC): separates and analyzes compounds that can be vaporized without decomposition.
  • GC/Mass Spectrometry: instrumental technique, comprising a gas chromatograph (GC) coupled to a mass spectrometer (MS), by which complex mixtures of chemicals may be separated, identified and quantified
  • Electro-Spray Mass Spectrometry: soft ionization technique typically used to determine the molecular weights of proteins, peptides, and other biological macromolecules

Specific equipment within CMBB:

Hewlett Packard HP 5890 Series II Gas Chromatograph

Waters 600 HPLC